In Shakespeare's Macbeth, the characterization of Macbeth and Lady Macbeth both establishes and reflects the themes of the play. I'll cite two examples.
The Macbeths are shown to be ambitious. Their ambition drives the plot and establishes the theme of ambition.
The Macbeths reverse gender roles. He feels pity and guilt and changes his mind about killing Duncan, and has to be talked back into going ahead with it. Lady Macbeth pleads to be unsexed and turned into a man, figuratively speaking. The witches also suggest the theme of androgyny, though, so in this case the Macbeths contribute to and reflect the theme, though they do not initially introduce it.
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